Knowledge workers represent more than half of all employees in advanced economies. In general knowledge workers are people who, when working, use their brain more than their muscles. Agricultural and industrial worlds depended on the work of manual workers and the life standard of their inhabitants and the success of agricultural and industrial organizations grew due to the increase of manual worker productivity. Existing knowledge economy depends on the work of knowledge workers and the labour productivity they can achieve. Knowledge is a major resource and tool that knowledge workers use to create values. Therefore, the way work with knowledge is organized in an organization influences the productivity of knowledge workers. This article discusses the results of research on knowledge management in organizations in the Czech Republic. This research started at the University of Economics, Prague in 2004 and continues today.