This work discusses how knowledge gatekeepers' intervention in the process of innovations and technology transfer was developed and applied in the manufacturing, financial and R&D firms in Rwanda and Uganda. The study was based on 150 questionnaires distributed, and used to collect empirical data. Furthermore, 25 interviews were conducted within ten organizations and fifteen well known opinion leaders and recognised experts in the fields concerned by this study. In an examination of the role of gatekeeper of knowledge in knowledge transfer processes the study has found that knowledge gatekeepers maybe individual, a firm, or a department within an organization. Furthermore, individuals working as knowledge gatekeepers must adapt to the recipient culture and knowledge sharing practice if they are to effect change. For technological innovations and knowledge transfer, the study found that the role of gatekeepers can be a multidimensional one, ranging from being trust and relationship builders, ambassadors within and from originating firms to receiving firms, and flag bearers. On the basis of the evidences obtained, the study has found no evidence to suggest that, in a gatekeeper's intervention within the knowledge creation process, there are no evidences that, in case of a firm acting as a knowledge gatekeeper, may necessarily develop asymmetries as well as put in place destructive mechanisms to restrain potential competitors within the sector from developing similar products or that they would necessarily incorporate innovative SMEs in their network who may provide related products to the leading firm.